Microscopy & Microbiology Training

The correct use of the light microscope is one of the basic and essential techniques employed in the bioscience laboratory. A good understanding of the theoretical basis and practical applications of a basic light microscope forms a solid grounding on which to build working knowledge of more advanced microscopy techniques.

Microbiology is the study of microorganisms and analysis of bacteria is a major component of this scientific area, including in clinical laboratories. This practical will introduce the concept of aseptic techniques in a task to purify a bacterial sample using the “streaking plate” method. Further investigations will include utilising the Gram staining procedure, the Miles and Misra drop dilution method, determination of colony forming units and analysis using the light microscope. The associated calculation methods involved in these techniques will also be taught.

Aims

The broad aims of the course are to:

Microscopy:

• Introduce the binocular light microscope and prepare it for use to view a prepared sample slide.
• Introduce the principles of simple light microscopy and associated calculations.
• Introduce oil immersion light microscopy.

• Introduce aseptic techniques in the microbiology laboratory.
• Introduce basic bacterial purification and analysis, using staining methods and light microscopy.

Who is the Course For?

Biological and medical scientists who wish to learn basic light microscopy and microbiology techniques. No prior knowledge of these techniques will be required, and the purpose of the course is to offer a practical point of access to these techniques in a practical user-friendly form.

Learning Outcomes

Knowledge and understanding:
Microscopy:

• Prepare a binocular microscope for use.
• Make necessary adjustments to view a (prepared) sample slide.

Microbiology:

• Apply aseptic techniques in the microbiology laboratory.
• Use the “streaking plate” method to purify a sample of bacteria.
• Utilise light microscopy in the analysis of bacterial colonies.
• Use the Gram staining method to identify bacterial colonies.
• Cell enumeration: determination of colony forming units using serial dilutions, the spread plate method and the Miles & Misra drop dilution method.
• Perform all calculations associated with these techniques.

Course Tutor