Introduction to CellProfiler for image analysis

The ‘Introduction to CellProfiler for image analysis’ course is designed for research scientists who use microscopy and want to learn how to use Cellprofiler, a free open-source image analysis software used to quantify microscopy data.

Common uses are counting cells and nuclei, quantifying the signal of reporters in the segmented images, as well as measuring size distribution of segmented objects, and much more. Attendees do not require any computer programming or coding experience.

The course will be presented by Todd Fallesen, Principal Image Analyst in the Advanced Light Microscopy facility at the Francis Crick Institute. Todd will support students in understanding the concepts behind CellProfiler image analysis, and how to link together modules to implement their own Image Analysis pipelines.

By the end of the training, delegates will be able to:

• Understand when CellProfiler can be used;
• Understand objects and modules in CellProfiler;
• Prepare images for CellProfiler analysis;
• Segment objects, use Machine Learning Segmentation Maps and build a pipeline to relate objects inside of other objects (i.e. FISH inside a nucleus);
• Build and troubleshoot a CellProfiler pipeline.

This training event is open to a maximum number of 10 delegates. Interested candidates should apply for a place by the 28th of April. Applications will be reviewed by the lead educator and successful candidates will be invited to register. Registration fees are £60 (+VAT if applicable).